Analyzeinvitrosamplesformicronucleiusingyourownflowcytometer
EachMicroFlowInVitro-1,000/200Kitcontainsallofthequality-assuredsupplies&reagentstoanalyze1,000samplesin96wellplatesor200samplesinlargerformatplatesorflasks.MaybeusedwithavarietyofattachmentandsUSPensioncelllinesincludingL5178Y,CHO-K1,TK6,V79,WIL-2,andHepG2,amongothers.
TechnicalSpecifications
SizeofKit
1,000samples(96wellplateformat)or200samples(standardformat-i.e.,flasksorplateformatswithfewerthan96wells)
ShippingConditionsoftheKit
- Ambient
StorageConditionsofKitComponents
- Freezer(-20°C)
- Refrigerator(4°C)
- Ambient
What"sIncludedintheKit
- IncompleteLysisSolution1
Lysescellsafterstaining - IncompleteLysisSolution2
Lysescellsafterstaining - NucleicAcidDyeA
StainstheDNAofcellsundergoingapoptosisornecrosis - NucleicAcidDyeB
Stainsnucleiandmicronuclei - RNaseSolution
RemovesRNAsoNucleicAcidDyeBstainsonlynucleiandmicronuclei - 10xBuffer
Usedinthelysissolutions
AdditionalMaterialsRequired
- Celllineandcellculturematerials
- Freezer(-20°C)andrefrigerator(4°C)
- Centrifugeandothergenerallabsupplies
- Flowcytometercapableof488nmexcitation
- Lightsource(fluorescentpreferred)
- Ifusing96-wellplates,an8-channelbridgeandaspiratormanifold(availablefromV&PScientific)
Litron has spent the last 40 years providing critical data to pharmaceutical, medical device, government and contract research organizations around the world. With a laser focus on DNA damage and other toxic effects, we develop state-of-the-art flow cytometric methods to solve challenging problems for the toxicology industry.
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